Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIYVVYDSEGEHTKVLAEAIAEGARENDAAEVFIDHVDQADIRKLKDMDAIIWGCPGHFGTISSGLKTWIDR-LGYLWAEGELINKVGAVFCTTATTHGGLEMTMHNLITPMFHQGMIVVGLPGNVPENALYGSYYGAGVTCPVDSDELMSEEGIQLGRALGRRVSQVTGNLTAGQ 2ARK Chain:A ((6-172)) -KVLVIYDTRTGNTKKMAELVAEGARSLEGTEVRLKHVDEATKEDVLWADGLAVGSPTNMGLVSWKMKRFFDDVLGDLW--GEIDGKIACAFSSSGGWGGGNEVACMSILTMLMNFGFLVFGVTDYVGKK--FTLHYGAVV-----AGEPRSEEEKEACRRLGRRLAEWVAIFVDGR

General information: TITO was launched using: RESULT: Template: 2ARK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 987 -124489 -126.13 -749.93 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -126.13 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_2ARK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ARK-query.scw PDB file : Tito_Scwrl_2ARK.pdb: