Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSFLGLLKKDIKLSRMWLLVWICGIIFLLGTGHIIASRTKEPLVIFGFFVAVAFFLLFLSPVFVFYHLRKEGKSQLWLYNPNGGLWLFSSKLAASLLYQFVIQLALTAYGIWMYHMLSVKNLLEHQVDITSTVALLNMYGLISSLDMSVTVIVFWTVFHSLRNWRGMRWAAMVLLVAMWLFF---DEYIISPLVESQKHFWPVTVYCNFDFHFHNVWRLELKPIHLSVLGFPIAIVITFLLLIMASKLLDRKVEV- 1EJH Chain:A ((5-190)) ---------------------------------------------------EHYIKHPLQNRWALWFFKNDK--SKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEK---------NKRG----------GRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKER--LGLPPKIVIGYQSHADTAT---KNRFVV

General information: TITO was launched using: RESULT: Template: 1EJH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 821 -126491 -154.07 -718.70 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -154.07 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.120

(partial model without unconserved sides chains): PDB file : Tito_1EJH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EJH-query.scw PDB file : Tito_Scwrl_1EJH.pdb: