Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGIAGTFIFMIVIGAAIGAVTNHLAIQMLFRPYKAYYLFGKRVPFTPGLIPRRRDELAKQMGLMVVNHLLTPEGIKKRLVSDAAKTQALRVGEQLIQKL-SLSEVTVKEALEKAGMKRPEKAADAWISSWTDDKLHELFRQYGDQSLKELVPIEVQEKLEEKIPMISGYILSRSVRYFESDEGKIRLGNMIDDFLKERGMLGSMVQLFLGNSSLADRVLPELLKFLRNEETNKLLSDLLKNEWGKLREYTFNEADEKWNAKALIFSLKRRVLQAFSTAPFFNNTIGTLTVRYESELTQQMLPALLDKLLEGISSNLESVLKRLRLEEIVKEQVDQFPVERLEEMVLSISKKEFKMITYLGGLLGGIIGAIQALFVILF 4JLT Chain:A ((285-372)) -------------------------------------------------------------------------------------STTLRYGFLLMLKYPHVTERVQKEIEQVIGSHRPPALDDRAKMPYTDAVIHEI------QRLGDLIPFGVPHTVTKDT-QFRGYVIPKNTEVF--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4JLT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 173 -7946 -45.93 -93.48 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -45.93 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_4JLT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JLT-query.scw PDB file : Tito_Scwrl_4JLT.pdb: