Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEVGILPVHVLYVFFIGAIILFMLLRKDTTFISLFGIFIISLWASHSLSASVSSLFHSFIYAAGELLPTIFIICFIVSMSDLLTKTGINEAMISPFASLVRGPVTAYWLIGGLMFAISLFFWPS----PGVALIGAVLLPAAARAGLTPLAAAMAMNLFGHGFALSGDFVIQAAPKLTADAAGIPVGDVVSASIPLVLIMGVTTTTAAFIMIQRERKKQEHPTSFSPVLSGEQDNSLYLPKRLRSILAFLIPLAFLADIACMLLFNLQGNDATALIGGSAICILFTVHFFVYKHK----GLEKITGYFIDGFKFGFKVFGPVIPIAAFFYLGDSGYESILGTSLPKGSHGIVNDLGIALSHMM--PMSKELAATALTAAGAITGLDGSGFSGISLAGSIAKLFSSALHADPAILTALGQISAIWVGGGTLVPWALIPAAAICKVDPF----ELARKNFIPVAIGLLVTTLAAVMML 4UUW Chain:A ((1-394)) MERAEILGV--------GTELLYGE-TLDT-----NTAEIARSLKPYALKVERTLRVADEVAPLAREVEEAFARARLVVLSGGLGPTP-DDVTREAVALALGEPLELD---EAVLGEIEAFFRARGRAMPEANRKQAMRIPSATWLK-NPRGTAPGWWVRKGG----KDLVLLPGP----PPEWRPMWQEVLPRLGLP-----RRPYAERVLKTWGIGESEIVERLGPLFVREEEVEVGTYPKVHGVEVVVRGR---EDRVAELAERIKKKLLKEVWGEGEMTLAEAVKRRMEREGATLSTMESLTGGLLGAEITR-------VPGASRFYLG----GVVSYSVGAKARFGVPQDL---LSRTVSAETARAMAEAARSLFGSTYALATTGVAG----------------PDPLEGEPPGTVYVALAG----------PTGAEVRRYRFPGDRETVRLRSVYAALALLVT--------

General information: TITO was launched using: RESULT: Template: 4UUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2011 -233992 -116.36 -615.77 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -116.36 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.152

(partial model without unconserved sides chains): PDB file : Tito_4UUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UUW-query.scw PDB file : Tito_Scwrl_4UUW.pdb: