Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKAKITAIGTYAPSRRLTNADLEKIVDTSDEWIVQRTGMRERRIADEHQFTSDLCIEAVKNLKSRYKGTLDDVDMILVATTTSDYAFPSTACRVQEYFGWESTGALDINATCAGLTYGLHLANGLITSGLHQKILVIAGETLSKVTDYTDRTTCVLFGDAAGALLVERDEETPGFLASVQGTSGNGGDIL---YRAGLRNEINGVQLVGSGKMVQNGREVYKWAARTVPGEFERLLHKAGLSSDDLDWFVPHSANLRMIESICEKTPFPIEKTLTSVEHYGNTSSVSIVLALDLAVKAGKLKKDQIVLLFGFGGGLTYTGLLIKWGM 4YLT Chain:A ((4-316)) ----KILGTGSYLPVQVRSNADLEKMVDTSDEWIVTRTGIRERRIAGLDETVATMGFQAAEKALEMAGIDKDDIGLIIVATTSSSHAFPSSACQVQRMLGIKDAASFDLAAACAGFTYALSVADQYVKSGAVKHAIVIGSDVLSRALDPEDRGTIILFGDGAGAVVLGASEQ-PGIMSTHLHADGRYGELLALPYP------PAYVTMAGN--------EVFKVAVTELAHIVDETLQVNNLDRTALDWLVPHQANLRIISATAKKLGMGMDKVVITLDRHGNTSAASVPSAFDEAVRDGRIQRGQLVLLEAFGGGFTWGSALVRF--

General information: TITO was launched using: RESULT: Template: 4YLT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2062 -241088 -116.92 -793.05 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -116.92 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_4YLT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YLT-query.scw PDB file : Tito_Scwrl_4YLT.pdb: