Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENQEVVLSIDAIQEPEQIKFNMSLKNQSERAIEFQFSTGQKFELVVYDSEHKERYRYSKEKMFTQAFQNLTLESGETYDFSDVWKEVPEPG-TYEVKVTFKGRAENLKQVQAVQQFEVK 4R4G Chain:A ((36-114)) -------------DDQLVLKVNVAVKNTGKDPLNVD--S---MDFTLYQGDTKMSDTDPE--DYSEKLQGSTINADKSVEGNLFFV--VDKGKQYELNYTP-------------------

General information: TITO was launched using: RESULT: Template: 4R4G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 289 -16215 -56.11 -207.88 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -56.11 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_4R4G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R4G-query.scw PDB file : Tito_Scwrl_4R4G.pdb: