Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRHVFLFLSQNKTLTKFAKAYGTRLGARRFVAGDTIESAVKTVKRLNRSGLCATIDYLGEYAASEKEANQVAEECKKAIQAIAEHQL------DSELSLKLTSIGLDLS--------EELALTHLRAILSVAKQYDVAVTIDMEDYSHYEQTLSIYRQCKQE-----FEKLGTVIQAYLYRAAEDIKKMRDLKPN------LRLVKGAYKES-----------AAVAFPDKRGTDLHFQSLIKLQLLSGN--YTAVATHDDDIIKFTKQLVAEHRIPASQFEFQMLYGIRPERQKEL------AKEGYRMRVYVPYGT--DWFSYFMRRIAERPANAAFVLKGILKK 2FZN Chain:A ((168-482)) ------------------------RLMGEQFVTGETIAEALANARKLEEKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALHPRYSRAQYDRVMEEL-YPRLKSLTLLARQYDIGINIDAEESDRLEISLDLLEKLCFEPELAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGLEGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPG-QYEFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSFV-------

General information: TITO was launched using: RESULT: Template: 2FZN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1360 -157968 -116.15 -587.24 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -116.15 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_2FZN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FZN-query.scw PDB file : Tito_Scwrl_2FZN.pdb: