Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHLLNPKAREIEISGIRKFSNLVAQHEDVISLTIGQPDFFTPHHVKAAAKKAIDENVTSYTPNAGYLELRQAVQLYMKKKADFNYDAESEIIITTGASQAIDAAFRTILSPGDEVIMPGPIYPGYEPIINLCGAKPVIV--DTTSHGFKLTARLIEDALTPNTKCVVLPYPSNPTGVTLSEEELKSIAALLKGRNVFVLSDEIYSELTYDRPHYSIATYLRDQTIVINGLSKSHSMTGWRIGFLFAPKDIAKHILKVHQYNVSCASSISQKAALEAVT----NGFDDALIMREQYKKRLDYVYDRLVSMGLDVVKPSGAFYIFPSIKSFGMTSFDFSMALLEDAGVALVPGSSF--STYGEGYVRLSFACSMDTLREGLDRLELFVLKKREAMQTINNGV 1V2D Chain:A ((3-368)) ----LHPRTEAA---IFPRMSGL-AQRLGAVNLGQGFPSNPPPPFLLEAVRRALGRQ-DQYAPPAGLPALREAL------AEEFAVEPES-VVVTSGATEALYVLLQSLVGPGDEVVVLEPFFDVYLPDAFLAGAKARLVRLDLTPEGFRLDLSALEKALTPRTRALLLNTPMNPTGLVFGERELEAIARLARAHDLFLISDEVYDELYYGERPRRLREFAPERTFTVGSAGKRLEATGYRVGWIVGPKEFMPRLAGMRQWTSFSAPTPLQAGVAEALKLARREGFYEAL--REGYRRRRDLLAGGLRAMGLRVYVPEGTYFLMAELP--GWDAFR----LVEEARVALIPASAFYLEDPPKDLFRFAFCKTEEELHLALERLGRVV--------------

General information: TITO was launched using: RESULT: Template: 1V2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1959 -252741 -129.02 -711.95 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -129.02 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_1V2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V2D-query.scw PDB file : Tito_Scwrl_1V2D.pdb: