Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKALKALGVLTTFVMLIVLIGGALVTKTGSGQGCGRQWPLCHGRFFPELNPAS--IIEWSHRFASGISIILVLSLAFWSWRKITPIFRETTFLAIMSIIFLFLQALLG---ALAVVFGSNALIMALHFGISLISFASVLILTLLIFEADKSVRTLVKPLQIGKKMQFHMIGILIYSYIVVYTGAYVRHTES---SLACPNVPLCSPLNNGLPT--QFHEWVQMGHRAAALLLFVWIIVAAVHAITSYKDQKQIFWGWISCLIFITLQA---LSGIMIVYSELALGFALAHSFFIACLFG----VLCYFLLLIARFRYESRQS 5DWZ Chain:A ((1-278)) M--LIQAVGVNLP---------PSYVCLEGPLGG-ERPRAQGDEMLMQRLLPAVREALDEAAVKPEEIDLIVGLAL--------SPDHLIENRDIMAPKIGHPLQKVLGANRAHVFDLTDSSLARALYVVDTLASDQGYRNVLVVRGESSQGLEV--------DSESGFALADGALALLCRPTGKAAFRRGALGGDPAQEWLPLSIPLNTDIRQVGDVKGHLNLPAQPG-LPEAVRAGFTRLAGDFPQLNWVREEW-------FGQGRPDGRCLGPFELASQLRAAQRDRLDELLLISFDPFGMVVEGVTLELA---------

General information: TITO was launched using: RESULT: Template: 5DWZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1529 -240898 -157.55 -922.98 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -157.55 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.143

(partial model without unconserved sides chains): PDB file : Tito_5DWZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DWZ-query.scw PDB file : Tito_Scwrl_5DWZ.pdb: