Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRVLFSVIVFTAVGFTFCQSKAHALTFTVLPITQKTDQWSVKVSEAKNVKEFTRPHKGEYQVYSLEVKNIGEKAATVDVQLYRNDPNSITRFSLFGCPDENCVKPKEDSKMLAES-L--NDG-------SPLKFN----HFMLADKASELEVVIIWTQKGQEGRNLKQTFKFTEDGVN 3WVF Chain:A ((65-195)) ------------------------------------------------------------TDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLETS-PQF-------IYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDA--AGNTFTKTFVLKRG---

General information: TITO was launched using: RESULT: Template: 3WVF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 277 390 1.41 4.24 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 1.41 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.345

(partial model without unconserved sides chains): PDB file : Tito_3WVF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WVF-query.scw PDB file : Tito_Scwrl_3WVF.pdb: