Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMAYEEYMRQLVVPMRRELTGAGFEELTTAEEVENFMEKAEGTTLVVVNSVCGCAAGLARPAATQAVLQNDKTPDNTVTVFAGQDKEATAKMREYFTGQEPSSPSMALLKGKEVVHFIPRHEIEGHDMEEIMKNLTAAFDAHC 3FHK Chain:E ((4-147)) MSMAYEEYMRQLVVPMRRELTGAGFEELTTAEEVENFMEKAEGTTLVVVNSVCGCAAGLARPAATQAVLQNDKTPDNTVTVFAGQDKEATAKMREYFTGAAPSSPSMALLKGKEVVHFIPRHEIEGHDMEEIMKNLTAAFDAHC

General information: TITO was launched using: RESULT: Template: 3FHK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 704 -60150 -85.44 -417.71 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain E : 0.99 3D Compatibility (PKB) : -85.44 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.693

(partial model without unconserved sides chains): PDB file : Tito_3FHK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FHK-query.scw PDB file : Tito_Scwrl_3FHK.pdb: