Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIGIFGGTFDPPHNGHLLMANEVLYQAGLDEIWFMPNQIPPHKQNEDYTDSFHRVEMLKLAIQSNPSFKLELVEMEREGPSYTFDTVSLLKQRYPNDQLFFIIGADMIEYLPKWYKLDELLNLIQFIGVKRPGFHVETPY-PLLFADVPEFEVSSTMIRERFKSKKPTDYLIPDKVKKYVEENGLYES 4S1O Chain:A ((5-200)) -RRLGVMGGTFDPIHYGHLVAASEVADLFDLDEVVFVPSGQP-------VSAAEHRYLMTVIATASNPRFSVSRVDIDRGGPTYTKDTLADLHALHPDSELYFTTGADALASIMSWQGWEELFELARFVGVSRPGY------DALTLVEIPALAISSTDCRQRAEQSRPLWYLMPDGVVQYVSKRRLY--

General information: TITO was launched using: RESULT: Template: 4S1O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 885 -87690 -99.08 -506.88 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -99.08 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_4S1O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4S1O-query.scw PDB file : Tito_Scwrl_4S1O.pdb: