Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKGQLFIGALTCLVASMSWGAMFPVADHALEFVDPFYFSFI-RYGVVTIMLVILLLVR-EGKKSFHLEGKAKWIILFGVMAFTIYNVLIFLGQRLMGKSGIMTASIAEAL-MP-------MLSIVILWGYKHVKPKKYTMISILIAFLGASMVITKGNISFFFSLGDHLFS--------------ILFIFIGVLGW---VVYTMGGQIFR-EWSTLRY-STLTCLFGTAIT-GIMTAILTAQGYVSVPSIKVI----AAIKYD-FL-----FMITLPG------IIALLSWNYGI---K-ILS-SING--ILFI------NFVPITT-L-------LIMVIKGYNITAFDIVGTLFV---------------IIGLILNNIYQRKEDYKQVLQKEKTNLTIT-- 4DIM Chain:A ((3-400)) DNKRLLILGA------GRGQLGLYKAAKEL----GIHTIAGTMPNAHKPCLNLADEISYMDISNPDEVEQKVKDLNLDGAATCCLDTGIVSLA-RICDKENLVGLNEEAAIMCGDKYKMKEAFKKYNVNTARHFVVRNENELKNALENLKLPVIVKATDLIYIAKKEEEAIDGFNETMNLTKRDYCIVEEFIEGYEFGAQAFVYKNDVLFVMPHGDETYMSHTAVPVGHYVPLDVKDDIIEKTKTEVKKAIKALGLNNCAVNVDMILKDNEVYIIELTGRVGANCLPELVEINYGIEYYKMIASMAISENPLVFWSQKSKENKAGLARMIIETEKSGILKEILNSNAKDDDIVEITFFKEENDEIKKFENSNDCIGQIIVKE-ETLDKCKDKLDVIINNINIILK

General information: TITO was launched using: RESULT: Template: 4DIM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1742 -103056 -59.16 -333.51 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -59.16 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.159

(partial model without unconserved sides chains): PDB file : Tito_4DIM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DIM-query.scw PDB file : Tito_Scwrl_4DIM.pdb: