Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDTIVIGAGQAGISIGYYLKQSD--QKFIILDKSHEVGESWKDRYDSLVLFTSRMYSSLPGMHLEGEKHGFPSKN-EIVAYLKKYVKKFEIPIQLRTEVISVLKIKNYFLIK-T---NREEYQTKNLVIATGPFHTPNIPSISKDLSDNINQLHSSQ-YKN-SKQLAYGNVLVVGGGNSGAQIAVELSK-ERVTYLACSNKLVYFPLMIGKRSIFWWFDKLGVLHASHTSIVGKFIQKKGDPVFGHELKHAIKQKEIILK--KRVIAAKQNEIIFKDSSTLEVNNIIWATGFRNPLCWINIK-GVLDQEGRIIHH-RGVSPVEGLYFIGLPWQHKR-------GSALLQGVGNDAEYIVKQMNGE 4EQS Chain:A ((1-310)) -PKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANC-ALP-------Y---VIGEVV--------EDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQFEESYDKLILSPGA--SANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVSLEVLENLYERGLHPTLIHRSD-KINKLM--DADMNQ------------------------------PILDELDKREIPYRLNEEINAINGNEITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIGDIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAG-

General information: TITO was launched using: RESULT: Template: 4EQS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1463 42204 28.85 146.03 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 28.85 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_4EQS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EQS-query.scw PDB file : Tito_Scwrl_4EQS.pdb: