Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTVHAKGNVLNKIGIPSHMVWGYIGVVIFMVGDGLEQGWLSPFLV------DHGLSMQQSASLFTMYGIAVTISAWLSGTFVQTW-GPRKTMTVGLLAFILGSAAFIGWAIPHM--YYPALLGSYALRGLGYPLFAYSFLVWVSYSTSQ--NILGKAVGWFWFMFTCGLNVLGPFYSSYAVPAFGEINTLWSALLFVAAGGILALFF-NKDKFTPIQKQDQ-PK---W-KELS-K-------------------------------------------------AFTIMFENPKVGI----GGVVKTINAIGQFGFAIFLPTYLAR-Y-----GYSVSEWLQIWGTLFFVNIVFNIIFGAVGDKLGWRNT------VMWFGGVGCGIFTLALYYTPQ------LIGHQYWVLMIIACCYGAALAGYVP-LSALLPTLAP-DNKGAAMSVLNLGSGLCAFIAPGIVSLFIGP--LGAGGVIWIFAALYFFSAFLTRFLTISEQSTDVYTEERFVRENVQTNFDKTVKQ 4W6V Chain:A ((10-491)) ---------GRTFFGHPYPLSGLFLSEMWERFSFYGIRPLLILFMAATVFDGGMGLPREQASAIVGIFAGSMYLAALPGGLLADNWLGQQRAVWYGSILIALGHLSIAL----SAFFGNDLFFIGLVFIVLGTGLFKTCISVMVGTLYKPGDARRDGGFSLFYMGINMGS-FIAPLLSGWLLRTHGWHWGFGIGGIGMLVALLIFRGFAIPAMKRYDAEVGLDSSWNKPT----GRWVTAIMAVVVVIIALISQGVIPINPVMIASLLVYVIAASVTLYFIYLFAFAKMSRKDRARLLVCFILLVSAAFFWSAFEQKPTSFNLFANDYTDRMVMGF-EIPTVWFQSINALFIILLAPVFSWA---------PSSITKFV-IGILCAAAGFAVMMYAAQHVLSSGGAGVSPLWLVMSILLLTLGELCLSPIGLATMTLLAPDRMRGQVMGLWFCASSLGNLAAGLIGGHVKADQLDMLPTLFARCSIALVICAAVL---------------------------------

General information: TITO was launched using: RESULT: Template: 4W6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1473 -182173 -123.67 -488.40 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -123.67 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.262

(partial model without unconserved sides chains): PDB file : Tito_4W6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4W6V-query.scw PDB file : Tito_Scwrl_4W6V.pdb: