Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQKRPKYMQIIDAAVEVIAENGYHQSQVSKIAKQAGVADGTIYLYFKNKEDILISLFKEKMGQFIERMEEDIKEKATAKEKLALVISKHFSLLAGDHNLAIVTQLELRQSNLELRQKINEILKGYLNILDGILTEGIQSGEIKEGLDVRLARQMIFGTIDETVTTWVMNDQKYDLVALSNSVLELLVSGIHNK 3ANP Chain:C ((6-194)) --QKKRRRERIFRAAMELFRNRGFQETTATEIAKAAHVSRGTFFNYYPYKEAVLLDYGSQLLAGLREEVRRLLAQGREPVEVLRHLFRVLAEGTAREKDLLLPMFYELLNPDPVRARAAF-EALPLGDLIAEILKPLREQGVLRQDFSLERMGRTLADLYFLSALRWAAYTPGRDLAEELEKNLRLLLEGML--

General information: TITO was launched using: RESULT: Template: 3ANP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 699 -107533 -153.84 -568.96 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -153.84 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.623

(partial model without unconserved sides chains): PDB file : Tito_3ANP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ANP-query.scw PDB file : Tito_Scwrl_3ANP.pdb: