Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILFAVSECTPFVKSGGLADVAGALPKALARLGNEVAVMLPKYSQIPEPWKKRMKKQAECTVAVGWRQQYCGIEHMAENDVNYYFIDNEYYFNRDSLYGHYDDG--ERFAFFSRA-VLEAAKVVNVQA--DIVHTHDWHTAMVNYLLKEEYRKHPFYERMKSVLTIHNLQFQGIFPPDVTHDLLGLEMDHFHYERLECNGFVNFMKAGIIAADHVTTVSPTYRNEIMTPYYGEQLEQVLQYREDDVTGILNGIDDTFYQPKSDPYIEAQYDSGDLACKLENKTKLQQRMGLPEKNDIPLISMVTRLTKQKGLDLVRR---IMHELLEEQDIQLVVLGTGEREFEDYFRYAEFAFHEKCRAYIGFDEPLAHQIYAGSDMFLMPSKFEPCGLGQLIALQYGAIPIVRETGGLYDTVRAYQEEEGTGNGFTFSAFNAHDLKFTIERALSFYCQQDVWK---SIVKTAMNADYSWGKSAKEYQRIFEQVTRSGRDVLE 3FRO Chain:A ((3-427)) MKVLLLGFEFLP-VKVGGLAEALTAISEALASLGHEVLVFTPSHG--------RFQGEEIGKIRVFGEEVQVKVSYEERGNLRIYRIGGGL-LDSEDVYGPGWDGLIRKAVTFGRASVLLLNDLLREEPLPDVVHFHDWHTVFAGALIKK-------YFKIPAVFTIHRLN-KSKLPAFYFHEAGLSELAPYPDIDPEHTG-------GYIA-DIVTTVSRGY----LIDEWG-----FFRNFEGKITYVFNGIDCSFW---NESYLTGSRD--------ERKKSLLSKFGMDEGVTFMFIGRFDR--GQKGVDVLLKAIEILSSKKEFQEMRFIIIGKGDPELEGWARSLEEKHGNVKVITEMLSREFVRELYGSVDFVIIPSYFEPFGLVALEAMCLGAIPIASAVGGLRDIIT-------NETGILVKAGDPGELANAILKALEL-SRSDLSKFRENCKKRAMS--FSWEKSAERYVKAY------------

General information: TITO was launched using: RESULT: Template: 3FRO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2328 -89966 -38.65 -217.31 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -38.65 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_3FRO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FRO-query.scw PDB file : Tito_Scwrl_3FRO.pdb: