Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence M--------KIRKANINTQTG----MITDVYLHENRKELRT---------LVAVPQLEWSTIISYE----EDKATLPERLEASLRRHTEETPAGELAKKIIHWVTEM------------ 1FHO Chain:A ((1-119)) MGDTGKLGRIIRHDAFQVWEGDEPPKLRYVFLFRNKIMFTEQDASTSPPSYTHYSSIRLDKYNIRQHTTDEDTIVLQPQEPGLPSFRIKPKDFETSEYVRKAWLRDIAEEQEKYAAERD

General information: TITO was launched using: RESULT: Template: 1FHO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 252 -2442 -9.69 -29.77 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -9.69 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.023

(partial model without unconserved sides chains): PDB file : Tito_1FHO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FHO-query.scw PDB file : Tito_Scwrl_1FHO.pdb: