Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSELFSVPYFIENLKQHIEMNQSEDKIHAMNSYYRSVVSTLVQDQLTKNAVVLKRIQHLDEAYNKVKRGESK 2DN9 Chain:A ((23-64)) ----------------------------EIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEV--

General information: TITO was launched using: RESULT: Template: 2DN9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 74 -9117 -123.20 -217.07 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -123.20 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.753

(partial model without unconserved sides chains): PDB file : Tito_2DN9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DN9-query.scw PDB file : Tito_Scwrl_2DN9.pdb: