Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHQIVTTQYGKVKG----TTENGVHKWKGIPYAKPPVGQWRFKAPEPPEVWEDVLDATAYGSICPQPSDLLSLSY--TEL--PRQ--SEDCLYVNVFAP-DTPSKNLPVMVWIHGGAFYLGAGSEPLYDGSKLAAQGEVIVVTLNYRLGPFGFLHLSSFNEAYSDNLGLLDQAAALKWVRENISAFGGDPDNVTVFGESAGGMSIAALLAMPAAKGLFQKAIMESGASR----TMTKEQAASTSAAFLQVLGINEGQ-----LDKLHTVSAEDLLKAADQLRIAEKENIFQLFFQPALDPKTLPEEPEKAIAEGAASGIPLLIGTTRDEGYLFFTPDSDVHSQ--ETLDAALEYLLGKPLA-EKVADLYPRSLESQIHMMTDLLF------WRPA---------VAYASAQ------SHYAPVWMYRFDWHPKK---PPYNKAFHALELPFVFG-NLDGLERMAKAEITDEVKQLSHTIQSAWITFAKTGNPST----EAVNWPAYHEETRETLILDSEITIENDPESEKRQKLFPSKGE 4EY6 Chain:A ((8-513)) ----LVTVRGGRLRGIRLKTPGGPVSAFLGIPFAEPPMGPRRFLPPEPKQPWSGVVDATTFQSVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLVWIYGGGFYSGASSLDVYDGRFLVQAERTVLVSMNYRVGAFGFLALPGSREA-PGNVGLLDQRLALQWVQENVAAFGGDPTSVTLFGESAGAASVGMHLLSPPSRGLFHRAVLQSGAPNGPWATVGMGEARRRATQLAHLVGCP---NDTELVACLRTRPAQVLVN--HEWHVLPQESVFRFSFVPVVDGDFLSDTPEALINAGDFHGLQVLVGVVKDEGSYFLVYGAPGFSKDNESLISRAEFLAGVRVGVPQVSDL---AAEAVVLHYTDWLHPEDPARLREALSDVVGDHNVVCPVAQLAGRLAAQGARVYAYVFEHRASTLSWPLWMGVPHGYEIEFIFGIPLD-----PSRNYTAEEKIFAQRLMRYWANFARTGDPNEPRDP---QWPPYTAGAQQYVSLD-----------------------

General information: TITO was launched using: RESULT: Template: 4EY6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2729 8312 3.05 18.68 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 3.05 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_4EY6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EY6-query.scw PDB file : Tito_Scwrl_4EY6.pdb: