Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEEKIYDVIIIGAGPAGMTAAVYTSRANLSTLMIERGIPGG-----QMANTEDVENYPGFES-ILGPELSNKMFEHAKKFGAEYAYGDIKEV-IDGKEYKVVKAGSKEYKARAVIIAAGAEYKKIGVPGEKELGGRGVSYCAVCDGA--FFKGKELVVVGGGDSAVEEGVYLTRFASKVTIVHRRDKLRAQSILQARAFDNEKVDFLWNKTVKEIHEENGKVGNVTLVDTVTGEESEFKTDGVFIYIGMLPLSKPFENLGITNEEGYIETNDRMETKVEGIFAAGDIREKSLRQIVTATGDGSIAAQSVQHYVEELQETLKTLK 4CBQ Chain:A ((3-312)) ----NIHDVVIIGSGPAAHTAAIYLGRSSLKPVMYEGFMAGGVAAGGQLTTTTIIENFPGFPNGIDGNELMMNMRTQSEKYGTTIITETIDHVDFSTQPFKLFTEEGKEVLTKSVIIATGATAKRMHVPGEDKYWQNGVSACAICDGAVPIFRNKVLMVVGGGDAAMEEALHLTKYGSKVIILHRRDAFRASKTMQERVLNHPKIEVIWNSELVELEGDGDLLNGAKIHNLVSGEYKVVPVAGLFYAIGHSPNSKFLGGQVKTADDGYILT-EGPKTSVDGVFACGDVCDRVYRQAIVAAGSGCMAALSCEKWLQ----------

General information: TITO was launched using: RESULT: Template: 4CBQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1781 49677 27.89 165.04 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 27.89 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_4CBQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CBQ-query.scw PDB file : Tito_Scwrl_4CBQ.pdb: