Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYAISSAADIWDHISESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCGSPASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 2WVJ Chain:A ((18-186)) ------RGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTR---------------ALPACLLRDVAQE-ALGVAVIGIDEGQFFP-DIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYSKRL------GTEKEVEVIGGADKYHSVCR-----------

General information: TITO was launched using: RESULT: Template: 2WVJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 811 -108049 -133.23 -697.09 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -133.23 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_2WVJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WVJ-query.scw PDB file : Tito_Scwrl_2WVJ.pdb: