Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRMLDVLMLVIGAFFFALAVNLFAIPNDLGEGGVTGITLILYYLFQWSPGVTNFILNAFLLLIGYKFLDGKTTVYTIIAVAANSLFLHLTHGWSIPSDELIINTIFAGVFAGVGIGMIIRVGGTTAGSAILARIANKYLDWNISYALLFFDLIVVFSSYFIIGAEKMMFTIVMLYIGTKVMDFIIEGLNTKKAITVISEN--KSEIAEQVNTLMDRGVTILSGKGNYTG---QSKEILYIVINKQELSMLKKIIRSCDK-----KAFVIVHDVRDVFGEGFVDISK 2GW8 Chain:A ((3-102)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGR-VDFLPKIKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI-------

General information: TITO was launched using: RESULT: Template: 2GW8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 274 5547 20.24 72.99 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.42 3D Compatibility (PKB) : 20.24 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.42 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_2GW8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GW8-query.scw PDB file : Tito_Scwrl_2GW8.pdb: