Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQHKKELKFYCIVTIPSAFVVLTVISFLLQEITFPVTASAFLNASWHNLLFLIPFGLFFYPVHIWMKREFGRWNDTEKKRG 1X2M Chain:A ((1-64)) GSSGSSGTAQ------------PNAILEKVFTAITKHPDEKRLEGLSKQL-------DWDVRSIQRWFRQRRNQEKPSGPSSG

General information: TITO was launched using: RESULT: Template: 1X2M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 149 -18434 -123.72 -288.03 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : -123.72 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.312

(partial model without unconserved sides chains): PDB file : Tito_1X2M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1X2M-query.scw PDB file : Tito_Scwrl_1X2M.pdb: