Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MN---ASTSPLPASDQTLRGILLICAAV--FLFASHDGLSKFL---SGFYPIVMVVWARYLVHTLLMVAVFVPRNGLAVIRSRRPL------MQLARALCLIGTSLFFTTGLRYLPLAE-------------------ATAVNFLAPLLVTALSVPLLKEKVSLGQWLAVLTGFIGVLFIVRPGGALF---------------TPAA---LFPLGSALC---FGLYQLLTRM-----LANSDS-----------PTTSNFLAGIFNTLIMSLLVPFFWQTPSLLHGLMMIALGGCGMGGHLLLTQAFRHAPPATLAPFSYGQIIFAGLLGLLVFGHVPDAWALLGILVICASGLVVAWRR--------GR---------------------------------------- 4KNC Chain:A ((15-437)) GNLCPAAAYDSRYNTKYLGFFTHLVQAQDDWLFRTTYDLRTDFGTSAEGWRELRALRDELKRKGIELVVVYQPTRGLVNREKLSPAEKAGFDYELAKKNYLATIARFRQAGIWTPDFSPLFDEKEEHAYYFKGDHHWTPHGARRSAKIVAETLKQVPGFEEIPKKQFESKRVGLLSKLGTFHKAAAQLCGNSYATQYVDRFETEPVGNPQIALVGTSNSGPAYNFAGFLEEFSGADILNNAVSGGGFDSSLLAYMTSEEFHKNPPKILIWEFATHYDMAQKSFYRQAMPLVDNGCSGRKTVLSRKVKLRQGRNEVLLNSAALPIRSGSYVADVTYSDPSVHELKNTIWYMNGRREQLKIEQSKAVDTGGRYVFQLRNDSDWADQQFLSLEIEAPDMPQGLEVQASICQAA

General information: TITO was launched using: RESULT: Template: 4KNC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -261224 for 1916 contacts (-136.3/contact) + 2D Compatibility (PS) -30737 + (NN) -8634 + (LL) 0 1D Compatibility (HY) 1600 + (ID) 2400 Total energy: -301395.0 ( -157.30 by residue) QMean score : 0.096

(partial model without unconserved sides chains): PDB file : Tito_4KNC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KNC-query.scw PDB file : Tito_Scwrl_4KNC.pdb: