Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKALKTLFIATALLGSAAGVQAADNFVGLTWGETSNNIQKSKSLNRNLNSPNLDKVIDNTGTWGIRAGQQ-FEQGRYYATYENISDTS-SGNKLRQQNLLGSYDAFLPIGDNNTKLFGGATLGLVKLEQDGKGFKRDSDVGYAAGLQAGILQELSKNASIEGGYRYLRTNASTEMTPHGGNKLGSLDLHSSSQFYLGANYKF 1P4T Chain:A ((3-155)) ---------------------ASGFYVQADAAHAKASSSL---------------GSAKGFSPRISAGYRIND-LRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQ-SPVKPYLGARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDAGYRYNYIGKVNT----------VKNV-RSGELSAGVRVKF

General information: TITO was launched using: RESULT: Template: 1P4T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) 48401 for 1133 contacts (42.7/contact) + 2D Compatibility (PS) -15190 + (NN) 547 + (LL) 2172 1D Compatibility (HY) -400 + (ID) 1350 Total energy: 34180.0 ( 30.17 by residue) QMean score : 0.096

(partial model without unconserved sides chains): PDB file : Tito_1P4T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P4T-query.scw PDB file : Tito_Scwrl_1P4T.pdb: