Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSKLIFFDIDGTIYDHD-KNIPESTRKTVAELQRQGHHVFIASGRSPFLVKPILE--EL-GIHSFISYNGQFVVFENQVIYKNPLPENAIRRLLKQADEGKHPVVFMAEDTMKATVADH-PHVLEGIGS-LKT---DYPETDDLFYEGKEIFQLLLFCQDEEEKAYAAF--PEFDLVRWHELSTDVLPHGGSKAEGIKKVIERLPFDIGDTYAFGDGLNDLQMIEYVGTGVAMGNAVPELKEIADFVTKPVDEDGIAYAVKELGLLK 2RBK Chain:A ((1-260)) -MTKALFFDIDGTLVSFETHRIPSSTIEALEAAHAKGLKIFIATGRPKAIINNLSELQDRNLIDGYITMNGAYCFVGEEVIYKSAIPQEEVKAMAAFCEKKGVPCIFVEEHN--ISVCQPNEMVKKIFYDFLHVNVIPTVSFEE--ASNKEVIQMTPFITEE-EEKEVLPSIPTCEIGRWYPAFADVTAKGDTKQKGIDEIIRHFGIKLEETMSFGDGGNDISMLRHAAIGVAMGQAKEDVKAAADYVTAPIDEDGISKAMKHFGI--

General information: TITO was launched using: RESULT: Template: 2RBK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1513 -151183 -99.92 -607.16 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -99.92 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_2RBK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RBK-query.scw PDB file : Tito_Scwrl_2RBK.pdb: