Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLVPRFRKQWFAYLTVLCLALAAAVSFGVPAKAAENPQTSVSNTGKEADATKNQTSKADQVSAPYEGTGKTSKSLYGGQTELEKNIQTLQPSSIIGTDERTRISSTTSFPYRATVQLSIKYPNTSSTYGCTGFLVNPNTVVTAGHCVYSQDHGWASTITAAPGRNGSSYPYGTYSGTMFYSVKGWTESKDTNYDYGAIKLNGSP--GNTVGWYGYRTTNSSSPVGLSSSVTGFPCDKTFGT-----MWSDTKPIRSAETY------------KL-----TYTTDTYGCQSGSPVYRNYSDTGQTAIAIHTNGGSSYNLGTRVTNDVFNNI---QYWANQ 1PQ7 Chain:A ((9-222)) ----------------------------------------------------------------------------------------------------------AGDFPFIV--SISR-----NGGPWCGGSLLNANTVLTAAHCVSGYAQ---SGFQIRAGSLSRTSGGITSSLSSVRVHPSYSG---NNNDLAILKLSTSIPSGGNIGYARLAASGSDPVAGSSATVAGWGATSEGGSSTPVNLLKVTVPIVSRATCRAQYGTSAITNQMFCAGVSSGGKDSCQGDSGGPIVDSS----NTLIGAVSWG---NGCARPNYSGVYASVGALRSFIDT

General information: TITO was launched using: RESULT: Template: 1PQ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1095 -104249 -95.20 -557.48 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -95.20 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_1PQ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PQ7-query.scw PDB file : Tito_Scwrl_1PQ7.pdb: