Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHQFSRNELAIGKEGLETLKNSTVAVLGVGGVGSFAAEALARSGVGRILLVDKDDVDITNVNRQLHALLSTVGQPKVDLMKARIADINPECEVIALKMFYTE-ETYEQFFDYGLDYVIDASDTICYKIHLMKECLKRDIPLISSMGAANKTDPTRFQIADISKTHTDPIAKVVRTKLRKEGIKKGVQVIFSDESPIVIREDVRKEVGNDEAKIRKAKMPPSSNAFVPSVAGLIMGGHVVMDLLKDIEIKRVKDK 1Y8Q Chain:B ((6-198)) ----------GLPRELAEAVAGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFYPKANIVAYHDSIMNPDYNVEFFR-QFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQ---VTT-IKKGVTECYECHP----------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1Y8Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 750 -67363 -89.82 -429.06 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -89.82 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_1Y8Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y8Q-query.scw PDB file : Tito_Scwrl_1Y8Q.pdb: