Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLVPRFRKQWFAYLTVLCLALAAAVSFGVPAKAAENPQTSVSNTGKEADATKNQTSKADQVSAPYEGTGKTSKSLYGGQTELEKNIQTLQPSSIIGTDERTRISSTTSFPYRATVQLSIKYPNTSSTYGCTGFLVNPNTVVTAGHCVYSQDHGWASTITAAPGRNGSSY----PYGTYSGTMFYSVKGWTESKDTNYDYGAIKLNGSP--GNTVGWYGYRTTNSSSPVGLSSSVTGFPCDKTFG----TMWSDTKPIRS---AE----------TYKL----TYTTDTYGCQSGSPVYRNYSDTGQTAIAIHTNGGSSYNLGTRVTNDVFNNI---QYWANQ 4H4F Chain:A ((10-235)) -----------------------------------------------------------------------------------------------------------HSWPWQISLQYLKN---DTWRHTCGGTLIASNFVLTAAHCISNTR-----TYRVAVGKNNLEVEDEEGSLFVGVDTIHVHKRWNAL-LLRNDIALIKLAEHVELSDTIQVACLPEKDSLLPKDYPCYVTGWGRLWTNGPIADKLQQGLQPVVDHATCSRIDWWGFRVKKTMVCAGGDGVISACNGDSGGPLNCQLENGSWEVFGIVSFG-SRRGCNTRKKPVVYTRVSAYIDWINE

General information: TITO was launched using: RESULT: Template: 4H4F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1093 -106361 -97.31 -542.66 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -97.31 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.277

(partial model without unconserved sides chains): PDB file : Tito_4H4F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H4F-query.scw PDB file : Tito_Scwrl_4H4F.pdb: