Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLVPRFRKQWFAYLTVLCLALAAAVSFGVPAKAAENPQTSVSNTGKEADATKNQTSKADQVSAPYEGTGKTSKSLYGGQTELEKNIQTLQPSSIIGTDERTRISSTTSFPYRATVQLSIKYPNTSSTYGCTGFLVNPNTVVTAGHCVYSQDHGWASTITAAPGRNGSSYPYGTYSGTMFYSVKGWTESKDTNYDYGAIKLNGSP--GNTVGWYGYRTTNSSSPVGLSSSVTGFPCDKTFG------TMW----------SDTKPIR-------------SAETYKLTYTTDTYGCQSGSPVYRNYSDTGQTAIAIHTNGGSSYNLGTRVTNDVFNNI---QYWANQ 2F9N Chain:A ((9-239)) ----------------------------------------------------------------------------------------------------------RSKWPWQVSLRVRD----RYWMHFCGGSLIHPQWVLTAAHCVGPDVK-DLATLRVQLREQHLYYQDQLLPVSRIIVHPQFYII-QTGADIALLELEEPVNISSRVHTVMLPPASETFPPGMPCWVTGWGDVDNDEPLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIIRDDMLCAGNSQRDSCQGDSGGPLVCKVN-GTWLQAGVVSWG---EGCAQPNRPGIYTRVTYYLDWIHH

General information: TITO was launched using: RESULT: Template: 2F9N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1030 -101608 -98.65 -515.78 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -98.65 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.258

(partial model without unconserved sides chains): PDB file : Tito_2F9N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F9N-query.scw PDB file : Tito_Scwrl_2F9N.pdb: