Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKHTAITTFILLGLTEDPQLQVLLFMFLFITYMLSVTGKLTIIALTMLDPHLKTPMYFFLQNLSFLEI-----SFTATCVPRFLYSISTGNKIITYNACVIQLFFADLFGVTEFFLLATMSYDRYVAICKPLHYMAIMSNKVCKTMVICCWMAALMIILPPLSLGFHLEFCDSNVINHFGCDALPILKIPCSDTSLIEQMVVASAVLTFIITLVCVVLSYTYIIRTILKFPSVQQKKKAFSTCSSHIT-----------VVSITYGSCIFIYIKPSAKEEVNINKGVSVLISSISPMLNSFIYTLRNEQVKQAFHDSLKKIAFRLKK 2I35 Chain:A ((33-315)) -----------------EPWQFSMLAAYMFLLIMLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTT-----LYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAF-TWVMALACAAPPL-VGWS-RYIPEGMQCSCGID----YYTPHEETN-NESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAVYNPVIYIMMNKQFRNCMVTTLCCGKNDDE-

General information: TITO was launched using: RESULT: Template: 2I35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -188601 for 2060 contacts (-91.6/contact) + 2D Compatibility (PS) -27030 + (NN) -6219 + (LL) 3016 1D Compatibility (HY) -24800 + (ID) 3200 Total energy: -246834.0 ( -119.82 by residue) QMean score : 0.215

(partial model without unconserved sides chains): PDB file : Tito_2I35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I35-query.scw PDB file : Tito_Scwrl_2I35.pdb: