Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHTPIGRLRTMGFIEGMSLLILLFIAMPLKYWAG--LP-----------------LAVTIVGSVHGGLFILYLLVLAYATFSVKWPLKWSAAGFIAAFVPFGNFLYDRGLRNYK 4WYF Chain:A ((131-223)) ---TPAG-LDTVFFSDSGSVSVEVAAKMALQYWRGRGLPGKRRLMTWRGGYHGDTFLAMSICDP-HGGMHSLWTDVLAAQVFAPQVPRDYDPA-YSAAF----------------

General information: TITO was launched using: RESULT: Template: 4WYF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 121 -24078 -198.99 -325.37 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -198.99 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.112

(partial model without unconserved sides chains): PDB file : Tito_4WYF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WYF-query.scw PDB file : Tito_Scwrl_4WYF.pdb: