Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHVLIAVILFFLSIGLSAGCAEAGNKTQNQSATEVNASPLQPAEYFIYHNLMNDKGLIKTDFSDQPSYLSESLGLWMEFLLSKNDAPHFQDQYQHLTDSFLM--------SNHLVTWKIQNGQ------ASGTNALIDDMRIMLSLDQAAAKWGRS---DYAQTARDIGTSLKTYNMNNGFFTDFYDSQAASKDVTLSYVMPDALAVLKKNG------IIDEETEQRNANVLYSAPL-KNG-----FLPKTYSSETK-----EYT---YDSEINLIDQLYAAWHLPEG----DEK------ASVLADWIKQEFQKN--GKLYGRYSAYTKEPAVQYESPSVYALAVLFLTKQHENSSVIKAIYDRMNDFEIHDPVKSYYGGYMSGTQTHSFDNLLPLLAERKL-FNENIIQ 1H12 Chain:A ((54-399)) -----------------------------------------TENGVYKAHYIK----AINPDE--GDDIRTEGQSWGMTAAVMLNKQEEFDNLWRFAKAYQKNPDNHPDAKKQGVYAWKLKLNQNGFVYKVDEGPAPDGEEYFAFALLNASARWGNSGEFNYYNDAITMLNTIKNKLMENQ-IIRFSPYI--DNLTDPSYHIPAFYDYFANNVTNQADKNYWRQVATKSRTLLKNHFTKVSGSPHWNLPTFLSRLDGSPVIGYIFNGQANPGQWYEFDAWRVIMNVGLDAHLMGAQAWHKSAVNKALGFLSYAKTNNSKNCYEQVYS-YGGAQNRGCAGEGQKAANAVALLAS-TNAGQANEFFNEFWSLSQPTG------------DYRYYNGSLYMLAMLHVSGNFKF--

General information: TITO was launched using: RESULT: Template: 1H12.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1556 -79669 -51.20 -269.15 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -51.20 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_1H12.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H12-query.scw PDB file : Tito_Scwrl_1H12.pdb: