Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLFIQNDQQRQWMEKIGRIADEFQQTAAEDDEQGRFPAEKIQKLRDAGYTALTLPASHGGGGISVYDMLLFQERLARGDAPTA-LSIGWHLSVIGELGEGNSWDEDVFAFVAKEVQNGAVINRAATEAKTGSP-----TRGGRPGTHAVKKDGKWAVNGRKTFTTMSQALDYFLVTAWIED---KQTTGVFLIHKDD---PGLSIEETWDMMAMRATGSHDLVLNEVMLDENKLVELLQG-PRG-----AKPNGWLLHIPAIYLGVAQAARDYAVQFASEYSPNSLNGPIKNVPAVQQRTGEMELELLNARHFLFHIA---QLYDDPVRRPHLTSELGAAKHIVTNAALSVVDKAMRIVGAKSLERTNPLQRYYRDVRAGLHNPPMDDAVIHKLAAEAFES 5AF7 Chain:A ((23-360)) -----------------------FASTAVQTDLTEQYPWDNVAQLRDAGFMGMMLPTSVGGRGLSTLDTVIVIEEMAKACATMGRITVDSNLGAIGAITKYGS--EEQIKLAADLVLAGDKPAICISEPNAGSAASEMTTRADKNGDH-------YILNGEKYWITGGGVSKLHLIFARVFDDGVEQGIGAFITVLDDHGPEGLKVGRRLYAMGVRGIPETHLEFHDLKIHKSMMITFPDGLKRGFAALMSAYNAQRVGAGAVALGIAQCAFEEGVAYLKRR--EQFGRPLAEFQGLQWMVADMSVQLEAARLMLRSAAVSGETFPD-------INKAAQAKIFAAETANKVTNDALQFFGSSGYGRHNPMERHVRDAR-----------------------

General information: TITO was launched using: RESULT: Template: 5AF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1739 -144626 -83.17 -456.23 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -83.17 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_5AF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AF7-query.scw PDB file : Tito_Scwrl_5AF7.pdb: