Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIAFLNSLVVTSPGFYKAEKITLDEVKQWL------KHYDGRYKSFIGHKSTAQFLQKLLGIRIEQNRKTFRHMKYQKAICFSLYERYPENVLLTQRDLEKARYQFYLLTRLD 2VGN Chain:A ((208-259)) -----LKTIILCSPGFYA--KILMDKIFQYAEEEHNKKILDNKGMFFIAHCSTG-YLQGI------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2VGN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 113 -15478 -136.97 -336.47 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -136.97 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_2VGN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VGN-query.scw PDB file : Tito_Scwrl_2VGN.pdb: