Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTSNRRMGLFY--VITGATC----WGIGG--TVAKKLFQEYQIDVN-------WLVTTRLLTAGMLLLMLQ-LFRKDRSQVI-----EVWKHKASAGQLLIFGLLGMLAVQYTY-------MASIQHGN----------AAVATLLQYLSPVIVIIYTLL--------------RKQTVLTKQDIITVVLALVGCFFLLTNG---SMSQLSVPAAAVVWG-------VLSGFAAAFYTLYAVGLLNKFDS---LVVVGWAMIIGGF-----------------ALGFIHPPWQLD------------FQRLTAEAYAYILFVILFGTMIAFWFF--------IKSLESLSPKETSLLGSLEPLSAVV---------TTVVWLKAPFGSFQWIGAICIIGITLILA---------------------LHKEPSMESEKSILKIRNHANRDI------- 1NTH Chain:A ((2-458)) TFRKSFDCYDFYDRAKVGEKCTQDDWDLMKIPMKAMELKQKYGLDFKGEFIPTDKDMMEKLFKAGFEMLLECGIYCTDTHRIVKYTEDEIWDAINNVQKEFVLGT-GRDAVNVRKRSVGDKAKPIVQGGPTGSPISEDVFMPVHMSYALEKEVDTIVNGVMTSVRGKSPIPKSPYEVLAAKTETRLIKNACAMAGRPGMGVKGPETSLSAQGNISADCTGGMTCTDSHEVSQLNELKIDLDAISVIAHYKGNSDIIMDEQMPIFGGYAGGIEETTIVDVATHINAVLMSSASWHLDGPVHIRWGSTNTRETLMIAGWACATISEFTDILSGNQYYPCAGPCTEMCLLEASAQSITDTASGREILSGVASAKGVVTDKTTGMEARMMGEVARATAGVEISEVNVILDKLVSLYEKNYASAPAGKTFQECYDVKTVTPTEEYMQVYDGARKKLEDLGLVF

General information: TITO was launched using: RESULT: Template: 1NTH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1624 -195015 -120.08 -613.25 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -120.08 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.153

(partial model without unconserved sides chains): PDB file : Tito_1NTH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NTH-query.scw PDB file : Tito_Scwrl_1NTH.pdb: