Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANGNKQAGQRISPSLTLLLATACGIIVANLYYAQPLVGLISKAIGLSPSGAGLIVTLTQIGYVAGLLFLVPLGDIIENKKLIVVSLLLSAAALTLTAF---VKHGTLFLAAAFFVGLGS-IAAQVLVPFASYLASDAARGRVVGNVMSGLLLGIMLSRPISSLVA--DIWGWNAIFALSAVVSVILAIVLSKVLPARKPTA--NTNYTA-------------------------------LLGSMWKLLRTTPVLRRRAIYHAFVFGA-----FSLFWTTVPLLLSGPDFHFSQKAIALY-ALVGIAGAVTAPIGGRLADRGLTRLATGIALGVVVVSLLLPLMIQS-S---SPVGITVLVAAAILLDMGVSANLVLSQRAIFS-LAPEIRSRLNGLFMAIFFLGGAIGSSIGGWAYASGGWSTALWIGIAFPVIALLYFITEK 4ZW9 Chain:A ((80-459)) ----------------------------------------------LLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSA-ALPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFK--DA--GVQEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKDNYNGMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYLGAYVFIIFTG--------------

General information: TITO was launched using: RESULT: Template: 4ZW9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1479 -233155 -157.64 -706.53 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -157.64 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_4ZW9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZW9-query.scw PDB file : Tito_Scwrl_4ZW9.pdb: