Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQRVSYYEIAPEGMKIMMDMEKYTKQSSINRTTRELIKIRVSQMNGCAFCIDMHTSDAR-KMGETEQRIYCLHAWNECDFYSPEEKAALELSEHITLIPSKRVPDELYHRVRE-HYDEEQYVDLVLIINQINSWNRISIAMGNRAASK 3LVY Chain:A ((55-198)) --NLIGLLANAPTALETYRTVGEINRRNSLTPTEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMAPDLLEALRNAT-PIDDDPKLDTLAKFTIAVINTK-GRVGDEAFADFLEVGYTPENALDVVLGVSLASLCNYANNMADTPINP-

General information: TITO was launched using: RESULT: Template: 3LVY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 585 -60902 -104.11 -428.89 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -104.11 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_3LVY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LVY-query.scw PDB file : Tito_Scwrl_3LVY.pdb: