Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIILVMFLLGIILGFIGAGGAGFVIALLTLLFHIPIHTALGTSLAGMAFTSLSGAYSHYREGNIQMKIGLIVGGFAAVGSFFGAKLTSFIPADLLHYLTAGMLFLSAILILIRLFILKEKAQVNQSTLSTYTRAVILGIAAGVLSGTFGIGSAPFIQIGLMIMLNLSIRHSVGTTMLVIIPLAVGGGIGYITEGFVDYVLLVKVLVGTMCGAYVGAKFTNLMPKVVLKSAIFLTPAIAGLLLLF 4QAS Chain:A ((5-201)) --------------------------SMFKLLLIFADPAEAARTLSLFPFSLNKENFYTYHTENVLLDVMV-LKTWGYRGVVQALSPPPSGYDLWINAGFAGAANPNIPLLKTYTITSVKELTPTTSVEEELEVTPIPRLPLAQLTSVRSPYRDGFHEH----------LQLVDMEGFFIAKQASLVACP---------CSMIKVSSNYT-TREGQDFLKNNKVKLSQKLAEAIFPIYSSFIDV-

General information: TITO was launched using: RESULT: Template: 4QAS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1070 -152826 -142.83 -775.77 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -142.83 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.174

(partial model without unconserved sides chains): PDB file : Tito_4QAS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QAS-query.scw PDB file : Tito_Scwrl_4QAS.pdb: