Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------MTDTRHMYGGPGFGHYQGFGIGHPGYGMQSTGYPGYGMYGGHP--GYGMQGYPDHGIHGGVGGYPGYGGY-----GGYPSGGYGGSPGTGSYPSMHHENDGHH----HYYHHHHDGKDNLHHHHHHVGKDNHHHHHDGHYGHHHHHMGHWGKDGYK--------------------------- 1FSZ Chain:? ((23-231)) SPEDKELLEYLQQTKAKITVVGCGGAGNNTITRLKME-GIEGAKTVAINTDAQQLIRTKADKKILIGKKLTRGLGAGGNPKIGEEAAKESAEEIKAAIQDSDMVFITCGLGGGTGTGSAPVVAEISKKIGALTVAVVTLPFVMEGKVRMKNAMEGLERLKQHTDTLVVIPNEKLFEIVPNMPLKLAFKVADEVLINAVKGLVELITKDGL

General information: TITO was launched using: RESULT: Template: 1FSZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 703 -12809 -18.22 -88.95 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -18.22 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.61 QMean score : -0.058

(partial model without unconserved sides chains): PDB file : Tito_1FSZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FSZ-query.scw PDB file : Tito_Scwrl_1FSZ.pdb: