Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDITPFLDKKSKTPLYEQLYTFFKQEISHARITKGTRLPSKRRLSSLLDVSTATIERAYEQLTAEGYVKSKPKIGWFAAEVEPGFPTAPDHFQQSVQPGLHQKNAPAIDFHQGSVDPTLFPFNAWRKSIVKSLDRYSGSFHTSGDP-QGEYELRHMIARFVRLSRGVNCEPGQIIIGAGTTVLLQNLC-LSLKPGTKIGFEEPGFHRSRRMFEANHMDVTPICSDAEG----VLPDELYRQNPYLMYTTPSHQFPIGTIMTITRRQELLAWAAETNSFIIEDDYDGEFRYSGHPIPSLQGLDPNGR---VIYLGTFSKSLLPSLRLSFMIVPPELMEPIQNNVQLMKQTVSAHSQLALADFIETGEWQKHINRMRSLYRKKHAVLLEAIRSELGNTVEILGKNSGLHILLRLLFPASEEEAIQAAADHGVTLYPVSPSYIEQTPFTSVLIGYGGLSEEDIRLGIQKLKTAWAPLISSY 2ZYJ Chain:A ((35-395)) -----------------------------------------------------------------------------------------------------------LSFAGGLPAPELFPKEEAAEAAARIL-REKGEVALQYSPTEGYAPLRAFVAEWI------GVRPEEVLITTGSQQALDLVGKVFLDEGSPVLLEAPSYMGAIQAFRLQGPRFLTVPAGEEGPDLDALEEVLKRERPRFLYLIPSFQNPTGGLTPLPARKRLLQMVMERGLVVVEDDAYRELYFGEARLPSLFELAREAGYPGVIYLGSFSKVLSPGLRVAFAVAHPEALQKLVQAKQGADLHTPMLNQMLVHELLKEG-FSERLERVRRVYREKAQAMLHALDREVPKEVRYTRPKGGMFVWMELPKGLSAEGLFRRALEENVAFVPGGPFFANGGGENTLRLSYATLDREGIAEGVRRLGRALKGLLA--

General information: TITO was launched using: RESULT: Template: 2ZYJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1967 -229953 -116.91 -653.28 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -116.91 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_2ZYJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZYJ-query.scw PDB file : Tito_Scwrl_2ZYJ.pdb: