Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAVCLVRHGETDWNLQQKCQGKTDIPLNATGERQARETGEYV--KDFSWDIIVTSPLKRAKRTAEIINEYL---HLPIVEMDDFKERDYGDAEGM--------------------------PLEERTKRYPDN--IY--------PNMETLEELTDRLMGGLAKV--NQAYPNKKVLIVAHGAAIHALLTEISGGDPE-LQSTRLVNACLSNIEFAEEKWRIKDYNINSHLSGFIK 3D8H Chain:A ((23-229)) ---LTLIRHGESEWNKENRFTGWTDVSLSEQGVSEAIEAGRMLLEKGFKFDVVYTSVLKRAIMTTWTVLKELGNINCPIINHWRLNERHYGALQGLNKSETASKFGEDQVKIWRRSFDVPPPVLEKSDPRWPGNELIYKGICPSCLPTTECLKDTVERVKPYFEDVIAPSIMSGKSVLVSAHGNSLRALLYLLEGMTPEQILEVNIPTAC---------------------------

General information: TITO was launched using: RESULT: Template: 3D8H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 828 -52571 -63.49 -322.52 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -63.49 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.436

(partial model without unconserved sides chains): PDB file : Tito_3D8H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D8H-query.scw PDB file : Tito_Scwrl_3D8H.pdb: