Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNVQEALNLLKENGYKYTNKREDMLQLFADSD-RYLTAKNVLSALNDDYPGLSFDTIYRNLSLYEELGILETTELSGEKLFRFKCSFTHHHHHFICLACGKTKEIESCPMDKLCDDLDG---YQVSGHKFEIYGTCPDCTAENQENTTA 2W57 Chain:A ((8-133)) ---------LKDAGLKVTLPRLKILEVLQQPECQHISAEELYKKLIDLGEEIGLATVYRVLNQFDDAGIVTRHHFEGGKSV-FELSTQHHHDHLVCLDCGEVIEFSDDVIEQRQKEIAAKYNVQLTNHSLYLYGKC-------------

General information: TITO was launched using: RESULT: Template: 2W57.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 412 -16955 -41.15 -138.97 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -41.15 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_2W57.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W57-query.scw PDB file : Tito_Scwrl_2W57.pdb: