Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIQDMILTLQKHWSSQGCVLMQAYDVEKGAGTMSPYTFLRSIGPEPWKVAYVEPSRRPADGRYGENPNRLYQHHQFQVIIK--PSPD---NIQELYLDSLRALGIDPLEHDIRFVEDNWENPSLGCAGLGWEVWLDGMEITQF----TYFQQVGGLECKPVSVEITYGIERLASYIQDKENVFDLEWTSGFTVKDLFMMAEYEHSVYTFETSDVDMLFQLFSTYEKEAIKQMDNGLVHPAYDYVLKCSHTFNLLDAKGAISVTERTGYIARVRNLARKVAKTYYEEREKLGFPMLKGEGSSHE 3DSQ Chain:A ((146-273)) -------------------------------------------WDKPIRIFEIGTCYRKESQ----GAQHLNEFTMLNLTELGTPLEERHQRLEDMARWVLEAAGIR----EFELVTESSVVYGDTV-----DVMKGDLELASGAMGPHFLDEKWEIF-DPWVG-LGFGLERLLMIREGTQHVQSM----------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3DSQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 509 -12544 -24.64 -105.41 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -24.64 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_3DSQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DSQ-query.scw PDB file : Tito_Scwrl_3DSQ.pdb: