Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATLSDVAKKANVSKMTVSRVINHPETVTDELKKLVHSAMKELNYIPNYAARALVQNRTQVVKLLILEEMDTTEPYYMNLLTGISRELDRNHYALQL--------VTRNSLNI---GQCDGIIATGLRKNDFEGVIKAFEK----PLVVFGQNEMGYDFIDV---NNEKGTFMATRHVMGLGEREVVFFGIDLDEPFER----AREQGYIRAMNKSFKK---SNMFRIDNSSKKSECLARELLKSMDNQAAFVCASDRIALGVIRAAQSLGKRIPEDVAVTGNDGVFLDRISSPRLTTVRQPVVEMGEACAKMLLKKMNEDGAAQGSLFFEPELIVRESTL 1ZAY Chain:A ((2-329)) --TIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELHYSPSAVARSLKVNHTKSIGLL---ATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLEKQRAYLSMMAQKRVDGLLV--MCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVIDNAFEGGYMAGRYLIERGHREI---GV-IPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQGDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDNVRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRS--

General information: TITO was launched using: RESULT: Template: 1ZAY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1549 -15284 -9.87 -50.44 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -9.87 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_1ZAY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZAY-query.scw PDB file : Tito_Scwrl_1ZAY.pdb: