Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLIVESPAKARTISKYLSKE-------------------FKVAASFGHVRDLPVKNG---------------SVDPDNDFAIKYEIIEKAEKYVKELVKEASQTSDIYLATDPDREGEAIAWNVIEALKERKAINDKSNIYRVVFNEITKRAVQEAVKNPREINMDLVRAQQARRALDYLVGFSLSPLLWTKLSGSK-SAGRVQSVALKLICEREDEISKFVSQEYWSIRAEIKDSKGETFFAMLSHYDNKKLEKFDIKNEEEAKGLVKQIESRQYAVSTVERKQVKRNPLPPFITSSLQQDAVNKLYFNVKNVMRIAQDLYEGIDIGGETVGLITYMRTDGFYIADEAVNSIRRSIKSLYGNKYLPKSSRQYVKKVKNAQEAHEAIRPT------DIDRTPDS---IKDYLTPEQFKLYGLIWKRTIASQME--SAILDQVVVEIS--STDQKVILRASGSIIFFDGFYKVYKNNVDGEDESMIPTMKEGEECKLISVDPEQHFTQPPSRYSEASIVKKMEEIGIGRPSTYAAIISVLQDREYVTLDNKRFIPSSRGKIVTIFLETFFQRCVEYDFTAQMEEKLDLISNGRADWKKELGYFWVPFFNHVNSVKQMTHDEIFNGIRDLVVDWFCSEEGKKEVNKKCPDCPDGILKLNFGKAGVFLGCSNYPTCTHTKEITGSNDNSEYPKSLGIDGVTEQEVVIKKGPFGLYLEFNSESEKKKTVSIPKDINVNDIDLSTATRLLSLPKVIGEHPETGKEVKIGLGRFGYYIFYDSKYFSLKKSFKEVLEIQLDEAVQIIANSPRKELKSLGFNEKGKEVFICNGRYGFYIKCGKTNVALGKNADIESVDLRRALELIKSKK 1GKU Chain:B ((507-1043)) -ALFIVESPTKARQISRFFGKPSVKVLDGAVVYEIPMQKYVLMVTASIGHVVDLITNRGFHGVLVNGRFVPVYASIK------------DNSRSRIEALRKLAHDAEFVIVGTDPDTEGEKIAWDLKNLLSGCGAVK------RAEFHEVTRRAILEALESLRDVDENLVKAQVVRRIEDRWIGFVLSQKLWERFNNRNLSAGRAQTLVLGWIIDRFQE-----SRERRKI-AIVRD------FDLVLEHDE---EEFDL------------------TIKLVEEREELRTPLPPYTTETMLSDANRILKFSVKQTMQIAQELFEN--------GLITYHRTDSTRVSDVG----QRIAKEYLGDDFV---GREW-----GESGAHECIRPTRPLTRDDVQRLIQEGVLVVEGLRWEHFALYDLIFRRFMASQCRPFKVVVKKYSIEFDGKTAEEERIVRAEGRA------YELYRA-VWVKNELPTGTFRVKAEVK--SVPKVLPFTQ-------SEIIQMMKERGIGRPSTYATIVDRLFMRNYVVEKYGRMIPTKLGIDVFRFLVRRYAKFVSEDRTRDLESRMDAIERGELDYLKALEDMYAEIKSID------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GKU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -148762 for 3724 contacts (-39.9/contact) + 2D Compatibility (PS) -48868 + (NN) -2985 + (LL) 18528 1D Compatibility (HY) -32800 + (ID) 9150 Total energy: -224037.0 ( -60.16 by residue) QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_1GKU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GKU-query.scw PDB file : Tito_Scwrl_1GKU.pdb: