Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MFLFTNGKVLWGAVIAAFILSIVFYPFLPTQMPIHYDV-----ANSPDLTVNKLAGTVM---LPVLMVVFAWARKINWQFVFAVYIL-----LICHIVVLCLAL 2E6J Chain:A ((1-112)) GSSGSSGPKIHFNFELLDIGKVFTGSAHCYEAILYNKGSIDALFNMTPPTSALGACFVFSPKEGIIEPSGVQAIQISFSSIILGNFEEEFLVNVNGSPEPVKLTIRGCVIGP

General information: TITO was launched using: RESULT: Template: 2E6J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 390 -106520 -273.13 -1170.54 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -273.13 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.181

(partial model without unconserved sides chains): PDB file : Tito_2E6J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E6J-query.scw PDB file : Tito_Scwrl_2E6J.pdb: