Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHVKALAIKGIMTIIVLYLVLGLGFGFTFGDTLLMTIVLGVISYLLGDLYVLPKWNNMIATLADFGLAFLVIWLMGMPLSMGMTGGEVALAALFGAIVMAIGEYCFHFYMMSKEIGKKHYLETRTDS 2W1B Chain:A ((341-404)) ---VKTLVEAIILVFLVMYLFLQ-----NFRATLIPTIAVPVV--LLGTF----------AVLAAFGFSINTLTMFGMVLAIGL--------------------------------------------

General information: TITO was launched using: RESULT: Template: 2W1B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 110 -29518 -268.35 -461.22 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -268.35 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.176

(partial model without unconserved sides chains): PDB file : Tito_2W1B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W1B-query.scw PDB file : Tito_Scwrl_2W1B.pdb: