Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFLHHILSTYASFF-DWKMWGEVLTDPVSWGLIGSLVVLEGLLSADNALVLAVMVKHLPEKQRKKALTYGLFGAYIFRFIFIGLGMLLIKFWWIKVLGALYLAWLVI------------KHFWIGEKEEEADGMKKNSWMVRT-----------FGIFWATVISVELMDLAFSVDSILAAFAVSEKVWVLLIGGMLGILMMRTVAKVFLVLID------------KIPELENTAFVLIGIIALKMAGSAFHYEM---PHSVFFIIIIAAFAVTLIIHYINKQKQVREQTAASKEE------------------ 4R1D Chain:B ((1-301)) MDKTGWITHCFGRFLIDLPPDAVINAGYYLWG--DRIEYLD-----DKPTELAARVDRLEQEWRTQ--RHKSKGNMFLRKIDFGNESVGLLSWSSEVASKTYLLDTYVTSKPTWHVYRWKGKVSVDREQHAVEISRALARNLRSRAPKEIPSEPGFCIDHAYIAGDSFQVERFGVGVTFPEHPGARFEFRSSTGAELNSLLERVDGFVQNMLSTFAGMETLRKGKHPVGSLPGEEYLVAGSDK-GQRGYTFMWEVQGKEESLTEPNLTAGLAV---LERSNENGKPPPPAFKSDKEALELWDTIVDSIRVRPTS

General information: TITO was launched using: RESULT: Template: 4R1D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 979 -123731 -126.38 -507.09 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -126.38 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.052

(partial model without unconserved sides chains): PDB file : Tito_4R1D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R1D-query.scw PDB file : Tito_Scwrl_4R1D.pdb: